optic atrophy protein 1 opa1  (Huabio Inc)

Journal: Genes & Diseases

Article Title: Tirzepatide, a dual GIP/GLP-1 receptor agonist, alleviates metabolic dysfunction-associated steatotic liver disease by reducing the expression of CD36 and OBP2A

doi: 10.1016/j.gendis.2025.101761

Figure Lengend Snippet: Oxidative phosphorylation was down-regulated after tirzepatide treatment. Transcriptomic sequencing was performed on liver tissues from three groups of mice: the NCD, HFFC, and HFFC with tirzepatide treatment groups. (A) Principal component analysis. (B) Volcano plot of differential expression. (C) KEGG enrichment analysis was performed on the differentially expressed genes between the HFFC group and the HFFC with tirzepatide treatment group. (D) Cluster analysis was performed on the genes involved in the reactive oxygen species and oxidative phosphorylation pathways across the NCD, HFFC, and HFFC with tirzepatide treatment groups. (E) Quantitative PCR was performed to validate the expression of genes in this pathway, including Atp5mc2, Gsta2, Nox4, Slc26a2, Hif1α, and Cycs. (F) Western blot analysis was used to detect Cyc protein expression in liver tissues from the NCD, HFD, HFFC, and HFD or HFFC with tirzepatide treatment groups. (G) Western blot analysis was used to detect the protein expression levels of ATP5A1, UQCRC1, SDHB, MTCO2, NDUFB8, and COX4 in liver tissues from the NCD, HFD, HFFC, and HFD or HFFC with tirzepatide treatment groups. (H) OPA1 protein expression was analyzed via western blotting. n = 5. The data are presented as mean ± SD.

Article Snippet: Optic atrophy protein 1 (OPA1) antibody (1:1000, cat. ET1705-9), COX IV antibody (1:2000, cat. ET1701-63), cytochrome C antibody (1:3000, cat. ET1610-16) and adipose triglyceride lipase antibody (1:1000, cat. HA721951) was obtained from HUABIO Biotechnology (Hangzhou, China).

Techniques: Phospho-proteomics, Sequencing, Quantitative Proteomics, Real-time Polymerase Chain Reaction, Expressing, Western Blot

Journal: International immunopharmacology

Article Title: Kaempferol improves mitochondrial homeostasis via mitochondrial dynamics and mitophagy in diabetic kidney disease.

doi: 10.1016/j.intimp.2025.115121

Figure Lengend Snippet: Fig. 4. Kaempferol improves mitochondrial dynamics and biogenesis in DKD rats. (A) Representative immunohistochemical staining images of dynamin-related protein 1 (Drp1), fission 1 (Fis1), mitofusin 1 (MFN1), and optic atrophy 1 (OPA1) (400× magnification). (B) Semiquantification of immunohistochemical stain ing for Drp1, Fis1, MFN1, and OPA1 expression (n = 6, data are presented as means ± SD). (C) Western blot analysis of protein expression levels of Drp1, Fis1, MFN1, and OPA1. (D) Relative expression levels of Drp1, Fis1, MFN1, and OPA1 (n = 4, data are presented as means ± SD). (E) Western blot analysis of PGC1α and TFAM expression levels. (F) Quantitative analysis of PGC1α and TFAM protein levels in different groups (n = 4, data are presented as means ± SD).

Article Snippet: Antibodies are as follows: Transforming growth factor-β (TGF-β) (21898–1-AP), α-smooth muscle actin (α-SMA) (14395–1-AP), collagen-IV (Col-4) (19674–1-AP), Bcl-2-associated X protein (Bax) (50599–2-Ig), B-cell lymphoma 2 (Bcl2) (26593–1-AP), Dynamin-1-like protein (Drp1) (12957–1-AP), fission 1 (Fis1) (10956–1-AP), Mitofusin-1 (MFN1) (13798–1-AP), Optic Atrophy 1 Protein (OPA1) (27733–1-AP), Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC1α) (66369–1-Ig), and β-actin (66009–1-Ig) were bought from Proteintech (Wuhan, China); microtubule-associated protein I light chain 3 (LC3) (ab192890), Beclin1 (ab207612), Autophagy-related 5 (ATG5) (ab108327), and p62 (ab109012) were bought from Abcam (Shanghai, China); Cysteineaspartic acid protease-3 (caspase-3) (9662) was bought from Cell Signaling Technology (Shanghai, China); Mitochondrial Transcription Factor A (TFAM) (A3173) was bought from ABclonal (Wuhan, China).

Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot

Journal: Journal of cellular physiology

Article Title: Augmented mitochondrial apoptotic signaling impairs C2C12 myoblast differentiation following cellular aging through sequential passaging.

doi: 10.1002/jcp.31155

Figure Lengend Snippet: FIGURE 4 High‐passage myoblasts display alterations in mitochondrial dynamics. Representative (a) immunoblot and quantification for (b) optic atrophy protein 1 (OPA1), (c) mitofusin 1 (MFN1), (d) mitofusin 2 (MFN2), and (e) dynamin 1 like (DNM1L) in proliferating (D0) and differentiating (D1, D3, D5) myoblasts.

Article Snippet: Membranes were then probed overnight at 4°C with primary antibodies against myosin heavy chain (MYH) (MF‐20, DSHB), myogenin (MYOG) (F5D, DSHB), BCL2‐associated X protein (BAX) (sc‐526, Santa Cruz), B cell leukemia/lymphoma 2 (BCL2) (sc‐7382, Santa Cruz), apoptosis‐inducing factor mitochondria associated 1 (AIFM1) (sc‐13116, Santa Cruz), cytochrome c (CYCS) (sc‐13156, Santa Cruz), voltage‐dependent anion channel 1 (VDAC1) (sc‐390996, Santa Cruz), adenine nucleotide translocator 1/2 (ANT1/2) (sc‐9299, Santa Cruz), endonuclease G (ENDOG) (ab9647, Abcam), superoxide dismutase 2 (SOD2) (SOD‐110, Stressgen), optic atrophy protein 1 (OPA1) (80471, Cell Signaling), mitofusin 1 (MFN1) (14739, Cell Signaling), mitofusin 2 (MFN2) (9482, Cell Signaling), dynamin 1 like (DNM1L) (8570, Cell Signaling), and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (2118, Cell Signaling).

Techniques: Western Blot